Contamination: The scourge of cell cultures worldwide

Elimination of Mycoplasma Contamination in Cell Cultures

These antibiotic treatments had a high efficiency of permanent cure: MRA 64%, ciprofloxacin 77%, BM-Cyclin 84%. Resistance to mycoplasma eradication was observed in some cell cultures: BM-Cyclin 5%, ciprofloxacin 14%, MRA 22%. Nearly all resistant contaminants that could be identified belonged to the species M. arginini and M. orale. Detrimental effects of the antibiotics were seen in form of c...

detection of pestivirus contamination in cell cultures by nested-pcr

in this study a nested-pcr assay was optimized for detection of two bvdv biotype of nadl strain. a part of 5' non-coding region of virus, 249 bp in size, was amplified in rt-pcr. pcr product was cloned in a ptz57r/t vector and sequencing results confirmed the specificity of the test. internal primers were designed and a 155 bp dna fragment was amplified in nested-pcr. the sensitivity of rt...

Contamination of blood cultures.

Workshop I: Mycoplasma Contamination and Infection of Cell Cultures

K. Yamamoto reviewed the many different procedures which have been employed in attempting to eliminate mycoplasmas from contaminated cell cultures. These have included antibiotics (tetracyclines, kanamycin, chloramphenicol, novobiocin, tylosin, and gentamicin). In addition, surface-active compounds such as triton-X or SPS, gold salts, photodynamic inactivation, and hyperimmune sera have been ut...

Treatment of Mycoplasma Contamination in Cell Cultures with Plasmocin

A high percentage of cell lines are chronically infected with various mycoplasma species. The addition of antibiotics that are particularly effective against these contaminants to the culture medium during a limited period of time is a simple, inexpensive, and very practical approach for decontaminating cell cultures. Here, we examined the effectiveness of the new antimycoplasma compound Plasmo...